Jan
31st
Thu
31st
Dynamics of picornavirus RNA replication
Replication of many picornaviruses is inhibited by low concentrations of guanidine. Guanidine-resistant mutants are readily isolated and the mutations map to the coding region for the 2C protein. Using in vitro replication assays it has been determined previously that guanidine blocks the initiation of negative-strand synthesis. This paper examines the dynamics of RNA replication, measured by quantitative RT-PCR, within cells infected with either swine vesicular disease…
